This field shows the epithelial lining of two adjacent villi. The columnar enterocytes show their TPA-positive elongated nuclei and, at their apex, the TPA-positive terminal web. This intra-cytoplasmic web (arrows) is composed of fine cytoskeletal filaments that insert on the junctional terminal bars. In this preparation the striated borders are unstained and not visible. The lateral cell walls are equally pale.

The apical pockets of mucigen granules (*) of goblet cells are unstained.

The TPA-positive threads in the lamina propria correspond to smooth muscle fibres (M).

Stain: TPA
Magnification: ×950