Contents Yves Clermont, Michael Lalli, Zsuzsanna Bencsath-Makkai
LIGHT-MICROSCOPIC HISTOLOGY ATLAS
     9. Skin
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    9.5   Epidermis Version française
Figure 9.5A Section of thick skin stained with TPA.

This method selectively stains the keratin filaments green. These filaments are abundant in the cytoplasm of keratinocytes, which explains the deep staining of the epidermis in this section.

Some basal cells contain melanin granules (arrows).

Figure 9.5B Higher magnification of a thin section of thick skin stained with TPA as in Figure 9.5A.

The keratin filaments are inserted on the desmosomes of keratinocytes and form spindles or spines (arrowheads) at the interfaces.

These spines seen at the interfaces of polyhedral keratinocytes are responsible for the term stratum spinosum given to this layer of the epidermis.

Stain: TPA
Magnification: ×900 (Fig. 9.5A); ×1200 (Fig. 9.5B)


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